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Research Interests

I am a Biophysicist. To find out more about biophysics:Biophysica l Society

Main Goal: Use light (including polarized light) to learn about biological structure and function.

Current interest: Study the effects of Nitric Oxide and Aggregation in biological systems, especially as it relates to diseases such as Sickle Cell Disease.

Current Group Members

Bradley Goetz, Undergraduate Student Researcher

Pamela Wang, Undergraduate Student Researcher

Ivan Azarov, Graduate Student

Tennille Presley Postdoctoral Researcher

Landon Bellavia, Graduate Student Researcher

Swati Basu, Research Assistant Professor

Michael Font, Undergraduate Student Researcher

Howard Shields, Emeritus Professor

Natalia Azarova, Undergraduate Student Researcher

Former Group Members

Virginia Lee Lockamy, Resident in medical Physics, U. Minn.

Tim Murphy, Medical Student, UNC

Dr. Kejing Chen, Johns Hopkins University

Dr. Xiuli Xu, National Institutes of Health

Dr. Svetlana Aroutiounian

Dr. Zhi "James" Huang, National Institutes of Health

Bharat Gummadi, Medical Student

Christopher Kolibash, Medical Student, WVU

Shirley Domingo, Medical Student, Wake Forest

Courtney Mull, Medical Student, UNC - Chapel Hill

Sara Smith, Medical Student, Wake Forest

Dr. Srishailkumar Hadimani, Scientist, Process Development Sigma - Aldrich Fine Chemicals
Dr. Joe Louderback, Research Scientist-Engineer, KLA-Tencor Bryan Lusk, Medical Student, Wake Forest Rupen Amin, Medical Student, Wake Forest

W. Lyle Gravatt, Business Man

Brian Cinc, Stock Market Genius

Alan Poole, MD/PhD Program at U. Texas Southwestern

Mansi Goyal

Chaicharn "Chai" Luangkamthorn

Dr. Fouad Azizi, Wayne State University Medical School

Erin Lichtenstein, Actress

Cynthia Irby, National Institutes of Health-> UAB Medical school, Pictured at right

Leigh Hearne, Business Woman, pictured at left.

Matt Frazier, Radford College.

Dr. Adedoyin Adeyiga, NC A&T University.

Jess McKay, Physics High School Teacher

Man Cho

Olutoyin "Toyin" Okanlawon, Fulbright fellow

Dr. Kris Huang, WFUSM Medical Student

Jared Hill, Undergraduate Student

Rachel Maree, Yale School of Public Health

Kevin Berardinelli, Georgetown University

Dr. Mahesh Joshi, University of Florida

Atul Mehta, Medical School

Dr. Xiaojun"Jessica"He, Ticona

Dr. Jinming Huang, University of Arkansas

Elizabeth Gordon, USMC Medical School

Anne Jeffers, Sarstedt, Inc

Dr. Lanlan Lin, CrackerJack Professor, Chinese Academy of Science

Selected Recent Publications:

Ivan Azarov, Xiaojun He, Anne Jeffers, Swati Basu, Burak Ucer; Roy R Hantgan, Andrew Levy, Daniel B. Kim-Shapiro. Rate of Nitric Oxide Scavenging by hemoglobin bound to haptoglobin. Nitric Oxide: Biology and Chemistry. 18, 296-302, 2008.

Xiaojun He, Ivan Azarov, Anne Jeffers, Tennille Presley, Jodi Richardson, S. Bruce King, Mark Gladwin, and Daniel Kim-Shapiro. The potential of Angeli's salt to decrease nitric oxide scavenging by plasma hemoglobin. Free Rad. Biol Med., 44, 1420-1432, 2008.

Rozalina Grubina, Swati Basu, Mauro Tiso, Daniel B. Kim-Shapiro, and Mark T. Gladwin. Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinity. J. Biol. Chem., 283, 3628-3638, 2008.

Swati Basu, Rozalina Grubina, Jinming Huang, Jeanet Conradie, Zhi Huang, Anne Jeffers, Alice Jiang, Xiaojun He, Ivan Azarov, Ryan Seibert, Atul Mehta1, Rakesh Patel, S. Bruce King, Neil Hogg, Abhik Ghosh, Mark T. Gladwin, Daniel B. Kim-Shapiro. Catalytic generation of N2O3 by a concerted nitrite reductase and anhydrase activity of hemoglobin. Nat. Chem Biol., 3, 785-794, 2007

Rozalina Grubina, Zhi Huang, Sruti Shiva, Mahesh S. Joshi, Ivan Azarov, Swati Basu,4, Lorna A. Ringwood, Alice Jiang, Neil Hogg, Daniel B. Kim-Shapiro, and Mark T. Gladwin. Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxy-hemoglobin. J. Biol. Chem., 282, 12916-12927, 2007

Kris T. Huang, Zhi Huang, Daniel B. Kim-Shapiro. Nitric Oxide Red Blood Cell Membrane Permeability at high and low Oxygen Tension. Nitric Oxide: Biology and Chemistry, 16, 209-216, 2007

Anne Jeffers, Mark T. Gladwin, and Daniel B. Kim-Shapiro. Computation of Plasma Hemoglobin Nitric Oxide Scavenging in Hemolytic Anemias. Free Rad. Biol. Med. 41, 1557-1565, 2006.

Mark T. Gladwin, Harold Raat, Sruti Shiva, Cameron Dezfulian, Neil Hogg, Daniel B. Kim-Shapiro, and Rakesh P. Patel. Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection and vasodilation, Amer. J. Physiol: Heart and Circ. Physiol. 291, 2026-2035, 2006.

Swati Basu, Jared D Hill, Howard Shields, Jinming Huang, S. Bruce King, Ph.D., Daniel B. Kim-Shapiro. Hemoglobin effects in the Saville Assay. Nitric Oxide: Biology and Chemistry. 15, 1-4, 2006.

Jinming Huang, Mamudu Yakubu, Daniel B Kim-Shapiro, and S. Bruce King. Rat Liver Mediated Metabolism of Hydroxyurea to Nitric Oxide. Free Rad. Biol. Med. 40, 1675-1681, 2006.

Daniel B. Kim-Shapiro, Alan N. Schechter, and Mark T. Gladwin. Unraveling the reactions of nitric oxide, nitrite and hemoglobin in physiology and therapeutics. Arteriosclerosis, Thrombosis, and Vascular Biol. 26, 697-705, 2006.

Kris T. Huang, Ivan Azarov, Swati Basu, Jinming Huang, Daniel B. Kim-Shapiro. Lack of Allosterically Controlled Intramolecular Transfer of Nitric Oxide from the Heme to Cysteine in the Beta Subunit of Hemoglobin. Blood 107, 2602-2604, 2006

Mark T Gladwin, Alan N Schechter, Daniel B Kim-Shapiro, Rakesh Patel, Neil Hogg, Sruti Shiva, Richard O Cannon, Malte Kelm, David Wink, Michael Graham Espey, Edward Oldfield, Ryszard M Pluta, Bruce Freeman, Jack Lancaster, Martin Feelisch & Jon O Lundberg,. The emerging biology of the nitrite anion. Nature Chemical Biology, 1, 308-314, 2005.

Ivan Azarov, Kris T. Huang, Swati Basu, Mark T. Gladwin, Neil Hogg, and Daniel B. Kim-Shapiro, Nitric Oxide Scavenging by Red Blood Cells as a Function of Hematocrit and Oxygenation. J. Biol. Chem, 280, 39024-39032, 2005.

Kris T. Huang, Agnes Keszler, Neil Patel, Rakesh P. Patel, Mark T. Gladwin, Daniel B. Kim-Shapiro, Neil Hogg, The reaction between Nitrite and Deoxyhemoglobin: Reassessment of the reaction kinetics and stochiometry, J. Biol. Chem., 280, 31126-31131, 2005.

Zhi Huang, Sruti Shiva, Daniel B. Kim-Shapiro, Rakesh P. Patel, Lorna A. Ringwood, Cythia E. Irby, Kris T. Huang, Chien Ho, Alan N. Schechter, Neil Hogg, and Mark T. Gladwin, Enzymatic nitrite reductase activity of hemoglobin generates NO under allosteric control, J. Clin. Invest., 115, 2099-2107, 2005

Anne Jeffers, Xiuli Xu, Kris Huang, Man Cho, Neil Hogg, Rakesh P. Patel, and Daniel B. Kim-Shapiro, Hemoglobin mediated nitrite activation of soluble guanylyl cyclase, Comp. Biochem. Physiol. Part A, 142, 130-135, 2005.

Fouad Azizi, Jerrold E. Kielbasa, Adedoyin M. Adeyiga, Rachel D. Maree, Matthew Frazier, Mamudu Yakubu, Howard Shields, S. Bruce King, and Daniel B. Kim-Shapiro, Rates of Nitric Oxide Dissociation from Hemoglobin, Free rad. Biol. Med., 39, 145-151, 2005.

Daniel B. Kim-Shapiro, Mark T. Gladwin, Rakesh P. Patel, and Neil Hogg, The reaction between nitrite and hemoglobin: The role of nitrite in hemoglobin-mediated hypoxic vasodilation, J. Inorg. Biochem. 99, 237-246, 2005.

Kejing Chen, Samir K. Ballas, Roy R. Hantgan, and Daniel B. Kim-Shapiro, Aggregation of Normal and Sickle Hemoglobin in High Concentration Phosphate Buffer. Biophys. J. 84, 4113-4121, 2004.

Daniel B. Kim-Shapiro, Rakesh P. Patel, Alan N. Schechter, Mark T. Gladwin, Richard O. Cannon, Neil Hogg, How do Red Blood Cells Dilate Blood Vessels?Reply. Circ. Res. 95, e10, 2004.

Christian J. Hunter, Andre Dejam, Arlin B. Blood, Howard Shields, Daniel B. Kim-Shapiro, Roberto F. Machado, Selamawit Tarekegn, Neda Mulla, Andrew O. Hopper, Alan N. Schechter, Gordon G. Power, and Mark T. Gladwin. Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator. Nat. Med., 10, 1122-1127, 2004.

Virginia L Lockamy, Howard Shields, Daniel B Kim-Shapiro and S. Bruce King. Iron Nitrosyl Hemoglobin Formation from the Reaction of Hydroxylamine and Hemoglobin under Physiological Conditions. Biochim. Biophys. Acta, 1674, 260-267, 2004.

Jinming Huang, Daniel B. Kim-Shapiro, S. Bruce King. Catalase-Mediated Nitric Oxide Formation from Hydroxyurea. J. Med. Chem., 47, 3495-3501, 2004.

Daniel Kim-Shapiro, Hemoglobin-nitric oxide cooperativity: Is NO the third respiratory ligand? Free Rad. Biol. Med. 36, 402-412, 2004

Kenyatta Cosby, Kristine S. Partovi, Jack H. Crawford, Rakesh P. Patel, Christopher D.Reiter, Sabrina Martyr, Benjamin K. Yang, Myron A. Waclawiw, Gloria Zalos, Xiuli Xu, Kris T. Huang, Howard Shields, Daniel B. Kim-Shapiro, Alan N. Schechter, Richard O. Cannon III, and Mark T. Gladwin. Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nat. Med. 9, 1498 1505, 2003.

Xiuli Xu, Man Cho, Netanya Y. Spencer, Neil Patel, Zhi Huang, Howard Shields, S. Bruce King, Mark T. Gladwin, Neil Hogg, and Daniel B. Kim-Shapiro, Measurements of nitric oxide on the heme iron and b -93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation. Proc. Natl. Acad. Sci. USA, 100, 11303-11308 2003.

Zhi, Huang, Leigh Hearne, Cynthia E. Irby, S. Bruce King, Samir K. Ballas, and Daniel B. Kim-Shapiro, Kinetics of Increased Deformability of Deoxygenated Sickle Cells Upon Oxygenation, Biophys. J., 85, 2003.

Virginia L. Lockamy, Jinming Huang, Howard Shields, Samir K. Ballas, S. Bruce King, and Daniel B. Kim-Shapiro, Urease enhances the formation of iron nitrosyl hemogolobin in the presence of Hydroxyurea, Biochim. Biophys. Acta, 1622, 109-116, 2003.

Xiuli Xu, Virginia L. Lockamy, Kejing Chen1, Zhi Huang, Howard Shields, S. Bruce King, Samir K. Ballas, James S. Nichols, Mark T. Gladwin, Constance T. Noguchi, Alan N. Schechter, and Daniel B. Kim-Shapiro, Effects of Iron Nitrosylation on Sickle Cell Hemoglobin Solubility, J. Biol. Chem. 277, 36787-36792, 2002.

Zhi Huang, K. B. Ucer, Timothy Murphy, , Richard T. Williams, S. Bruce King and Daniel B. Kim-Shapiro, Kinetics of Nitric Oxide Binding to R-state Hemoglobin, Biophys. Biochem. Res. Comm., 292, 812-818, 2002.

J. Huang, S. B. Hadimani, J. W. Rupon, S. K. Ballas, D. B. Kim-Shapiro and S. B. King. Iron Nitrosyl Hemoglobin formation from the reactions of Hemoglobin and Hydroxyurea, Biochemsitry 41, 2466-2474, 2002.

Z. Huang, J. G. Louderback,, F. Azizi, M. Goyal, S. Bruce King, and D. B. Kim-Shapiro. Nitric Oxide Binding to Hemoglobin in Oxygenated Blood. Biophy. Biochem. Acta. 1568 (3), 252-260, 2001.

Z. Huang, J. G. Louderback, S. B. King, S. K. Ballas and Daniel B. Kim-Shapiro. Hydroxyurea Reduces the Deformability of Sickle Red Blood Cells in Vitro. Am. J. Hematology 67, 151-156, 2001.

S. Kh. Aroutiounian, J.G. Louderback, S.K. Ballas, and D.B. Kim-Shapiro. Evidence for carbon monoxide binding to sickle cell polymers during melting. Biophys. Chem. 91, 167-181, 2001.

J. W. Rupon, S. R. Domingo, S. V. Smith, B. K. Gummadi, H. Shields, S. K. Ballas, S. B.King, and D. B. Kim-Shapiro. The Reactions of Myoglobin, Normal Adult Hemoglobin, Sickle Cell Hemoglobin and Hemin with Hydroxyurea. Biophys. Chem. 84 1-11 2000

D.B. Kim-Shapiro, S.B. King, H. Shields, C.P. Kolibash, W. Lyle Gravatt, and S.K. Ballas, The Reaction of Deoxy-Sickle Cell Hemolglobin with Hydroxyurea, Biochem. Biophys. Acta., 1428, 381-387, 1999.

J. G. Louderback, S. Kh. Aroutiounian, W. C. Kerr, S. K. Ballas, D. B. Kim-Shapiro. Temperature and Domain Size Dependence of Sickle Cell Hemoglobin Polymer Melting in High Concentration Phosphate Buffer. Biophys.Chem. 80 21-30 1999.

J.G. Louderback, S.K. Ballas, and D.B. Kim-Shapiro, Sickle hemoglobin polymer melting in high concentration phosphate buffer, Biophys. J.76, 2216-2222, 1999.

Y. Xu, C.D. Mull, C.L. Bonifant, G. Yasaki, E.C. Palmer, H. Shields, S.K. Ballas, D.B. Kim-Shapiro, and S.B. King, Nitrosylation of sickle cell hemoglobin by hydroxyurea, J. Org. Chem , 63, 6452-6453, 1998.

D.B. Kim-Shapiro, S.B. King, C.L. Bonifant, and S.K. Ballas, Time-resolved absor ption study of the reaction of hydroxyurea with Sickle Cell hemoglobin, Biochem . Biophys. Acta., 1380, 64-74, 1998.

D.B. Kim-Shapiro and P.G. Hull. Polarized Light Scattering from Sickle Cell Hemoglobin Polymers. J. Chem. Phys., 107, 1625-1630, 1997.

R.A. Goldbeck, L. Sagle, D.B.Kim-Shapiro, V. Flores, and D. S. K liger, Evidence for Heme-Heme Coupling in the Soret Circular Dichroism of Hemogl obin, Biochem. Biophys. Res. Comm., 235, 610-614, 1997.

D.B. Shapiro, R.M. Esquerra, R.A. Goldbeck, Samir K. Ballas, N. Mohandas and D.S. Kliger, Mechanism of Slow Religation to Sickle Cell Polymers Following Ligand Photolysis, J. Mol. Biol., 259, 947-956, 1996.

D.B. Shapiro, R.M. Esquerra, R.A. Goldbeck, Samir K. Ballas, N. Mohandas and D.S. Kliger, Carbon Monoxide Rebinding Kinetics to Hemoglobin S Polymers Following Ligand Photolysis, J. Biol. Chem., 270, 26078-26085, 1995.

D.B. Shapiro, R.A. Goldbeck, D. Che, R. M. Esquerra, S.J. Paquette, and D.S. Kliger, Nanosecond Optical Rotary Dispersion Spectroscopy, Appication to Photolyzed Hemoglobin-CO Kinetics, Biophys. J., 68 (1), 326-334, 1995.

D.B. Shapiro P.G. Hull, A.J. Hunt, and J.E. Hearst, Calculations of the Mueller scattering matrix for a DNA Plectonemic Helix, J. Chem. Phys., 101 (5), 4214-4221, 1994.

D.B. Shapiro, M.F. Maestre, W.M. McClain, PG. Hull, Y. Shi, M.S. Quinby-Hunt, J. E. Hearst, and A.J. Hunt, Determination of the Average Direction of DNA in the Octopus Sperm Head Eledone Cirhossa through Polarized Light Scattering, Appl. Opt., 33 (24), 5733-5744, 1994.