Mailing Address: Chemistry
Department, Wake Forest University, Winston-Salem, NC 27109. Research Interests Analytical ChemistryResearch interests center upon the utilization of organized assemblies (cyclodextrins, surfactant micelles and vesicles, liposomes, polymers, inverted micelles) to enhance chemical analysis and separation science techniques. Examples of specific projects include the use of such media to aid in the preservation of clinical and environmental samples. That is, the adsorption of analytes upon their container surfaces and/or their degradation/decomposition reactions can be greatly diminished in the presence of appropriate ordered media. Upon ionic strength or temperature alteration, aqueous solutions of some micelle and/or polymer solutions can phase separate, with most of the surfactant (or polymer) concentrated in the small volume element of the surfactant-rich (or polymer-rich) phase. Any analyte species that associates or binds to the micelle/polymer entity will thus be concentrated in that phase. Such phase behavior (clouding phenomenon) or ordered media systems can be employed to design environmentally friendly extractive / enrichment techniques. The continued use of such cloud point extraction procedures to extract target analytes from solid (soil, sludge, etc.) matrices is of particular interest. The addition of gelatin to some reversed micelle solutions above a critical temperature results in the formation of elastic (solid) gels, which still retain many of the general and beneficial features of reversed micelle solutions. The use of such so-called organogels for entrapment of enzymes for use in enantioselective organic transformations for the purposes of optical isomeric resolutions is under investigation. In addition, such gels can be used to sorb and concentrate desired analytes in a manner akin to extraction or enrichment steps prior to spectral or other analysis. Of particular interest is the use of such gels to concentration and detoxify substances of environmental concern (phenols, anilines, etc.) from aqueous or organic solutions. A new project concerns investigation of the feasibility of employing capillary electrophoretic techniques for the separation and identification of microbes (bacteria, fungi, yeast, virus). Difficulties to be overcome include the optimization of the microorganisim sample preparation protocol (to ensure the presence of intact, non-aggregated cell populations) and selection of an appropriate run buffer polymeric medium.
Publications Reversed Phase Liquid Chromatographic Method for the Separation and Determination of Positional Isomeric Mono- and Di-substituted Anilines and Phenols on a R,S-Hydroxypropyl Ether Beta-Cyclodextrin Column, V. Peno, A. M. Afonso, V. Gonzalez, and W. L. Hinze, Journal of Liquid Chromatography, 26 (#1), 2003, 1-15. Covalently Bound Ionene Polyelectrolyte-Silica Gel Stationary Phases for HPLC, Y. Suzuki, F. H. Quina, A. Berthod, R. W. Williams, M. Culha, I. U. Mohammadzai and W. L. Hinze, Analytical Chemistry, 73 (#8), 2001, 1754-1765. Bile Acid/Salt Surfactant Systems: General Properties and Survey of Analytical Applications, W. L. Hinze, W. Hu, F. H. Quina and I. U. Mohammadzai, in Organized Asssemblies in Chemical Analysis, Vol. 2, Bile Acid/Salt Surfactant Systems, W. L. Hinze, Ed., JAI Press, Inc.: Stamford, CT, 2000, Ch. 1, pp. 1-70. Fluorescence in Organized Assemblies, W. L. Hinze, in Encyclopedia of Analytical Chemistry, R. A. Meyers, Ed., John Wiley & Sons Ltd.: Chichester, 2000, pp. 10364-10447. Effect of a Variety of Organic Additives on Retention and Efficiency in Micellar Liquid Chromatography, S. Lopez-Grio, M. C. Garcia-Alvarez-Coque, W. L. Hinze, F. H. Quina and A. Berthod, Analytical Chemistry, 72 (#20), 2000, 4826-4835. Surfactant-Mediated Cloud Point Extractions: An Environmentally Benign Alternative Separation Approach, F. H. Quina and W. L. Hinze, Industrial & Engineering Chemistry Research, 38 (#11), 1999, 4150-4168.
|
